plv teto tfap2c addgene  (Addgene inc)

Journal: Scientific reports

Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

doi: 10.1038/s41598-025-03830-x

Figure Lengend Snippet: Fig. 4. Genetic depletion of TFAP2C in the initial phase of TSLC derivation (a) Schematic overview of molecular analyses of the first step of TSLC derivation. (b) Western blot analysis of TFAP2C in TFAP2C- knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5), with or without AP for 4 days. ACTIN was used as a loading control. (c) Flow cytometric analysis of GATA3 expression in TFAP2C-knockout clones (T2CKO#3 and #12) and the parental ESCs (G3KI#2–5). Wild-type KhES-1 cells were used as negative controls. (d) Immunofluorescence images of CTB-related markers in TFAP2C knockout clones (T2CKO#3) and parental ESCs (G3KI#2–5). Scale bars, 100 μm.

Article Snippet: Generation of TFAP2C knockout cell lines A set of guide RNAs(gRNAs) targeting TFAP2C was designed as illustrated in Supplementary Fig. S5, and the target sequences are shown in Supplementary Table S1. gRNA expression vectors were constructed using the Cas9 sgRNA backbone vector (Addgene, #68463).

Techniques: Western Blot, Knock-Out, Clone Assay, Control, Expressing, Immunofluorescence

Journal: Scientific reports

Article Title: Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability.

doi: 10.1038/s41598-025-03830-x

Figure Lengend Snippet: Fig. 6. Schematic summary of TSLC derivation from primed human ESCs. TSLC derivation from primed ESCs involves two distinct molecular programs: a BMP-dependent lineage conversion into the trophoblasts, followed by a TFAP2C-dependent acquisition of self-renewal capacity.

Article Snippet: Generation of TFAP2C knockout cell lines A set of guide RNAs(gRNAs) targeting TFAP2C was designed as illustrated in Supplementary Fig. S5, and the target sequences are shown in Supplementary Table S1. gRNA expression vectors were constructed using the Cas9 sgRNA backbone vector (Addgene, #68463).

Techniques: